NASC

NASC ID: N567603

Name: SALK_067603

ABRC stock number: SALK_067603

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - T20D1.20 R300-3' At5g19510 T20D1.30 Exon. At5g19500. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_067603.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G19510.1 (at chr 5 pos 6581904 (W/6581856-6581904) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 46, R 48, C 42 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366187.

SALKseq_067603.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G07410.2=P355 and AT4G07415.1 (at chr 4 pos 4205601 (W/4205524-4205601) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 46, R 48, C 42 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356145.

SALKseq_067603.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G55310.1 (at chr 5 pos 22433990 (W/22433929-22433990) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 46, R 48, C 42 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359491.

SALKseq_067603.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intron of AT5G62110.1 (at chr 5 pos 24944081 (C/24944081-24944146) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 46, R 48, C 42 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362843.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_067603

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654235 (SALK_067603C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G07410 AT4G07415 At5g12110 AT5G55310 AT5G62110 At5g19510

EMBL accession:

BH848177

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.