NASC

NASC ID: N568352

Name: SALK_068352

ABRC stock number: SALK_068352

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At1g53200 F12M16.11 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_068352.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G53200.1 (at chr 1 pos 19840830 (C/19840830-19840893) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 11, R 11, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413077.

SALKseq_068352.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G23420.1 (at chr 1 pos 8317223 (W/8317188-8317223) on the TAIR10). The sequences' 1-36 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 11, R 11, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342820.

SALKseq_068352.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G57030.1=P355 and AT3G57040.1=P355 (at chr 3 pos 21108139 (W/21108061-21108139) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 11, R 11, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346170.

SALKseq_068352.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G37450.1 (at chr 5 pos 14857076 (W/14857025-14857076) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 11, R 11, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349515.

SALKseq_068352.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G44180.2=UTR3 and AT5G44190.1=P355 (at chr 5 pos 17794201 (C/17794201-17794260) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 11, R 11, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352872.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_068352

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654239 (SALK_068352C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G23420 AT3G57030 AT3G57040 AT5G37450 AT5G44180 AT5G44190 At1g53200

EMBL accession:

BZ593298

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.