NASC

NASC ID: N570162

Name: SALK_070162

ABRC stock number: SALK_070162

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_070162.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G38510.2 (at chr 4 pos 18015168 (W/18015113-18015168) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 53-75 bps onto the T-DNA. Pools P 45, R 44, C 47 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376405.

SALKseq_070162.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G29840.1 (at chr 2 pos 12732715 (C/12732715-12732776) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 45, R 44, C 47 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369695.

SALKseq_070162.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G21216.1 and AT4G21220.1=UTR3 (at chr 4 pos 11315037 (W/11314976-11315037) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 45, R 44, C 47 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373056.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_070162

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654250 (SALK_070162C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G29840 AT4G21216 AT4G21220 At4g38510

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.