NASC

NASC ID: N570923

Name: SALK_070923

ABRC stock number: SALK_070923

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - MLE2.7 Intron. At5g63440. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_070923.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G63440.1 (at chr 5 pos 25407586 (W/25407533-25407586) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 52-74 bps onto the T-DNA. Pools P 47, R 47, C 42 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO359698.

SALKseq_070923.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G07025.1 (at chr 1 pos 2157827 (C/2157827-2157871) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 40-67 bps onto the T-DNA. Pools P 47, R 47, C 42 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO346324.

SALKseq_070923.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G18270.1 (at chr 1 pos 6288062 (C/6288062-6288131) on the TAIR10). The sequences' 1-70 bps mapped on the genome. Pools P 47, R 47, C 42 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO349672.

SALKseq_070923.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G24793.1 (at chr 1 pos 8768334 (C/8768334-8768384) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 47, R 47, C 42 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO353027.

SALKseq_070923.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G32820.1 (at chr 4 pos 15841949 (C/15841949-15842009) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 47, R 47, C 42 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO356350.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_070923

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_070923C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G07025 AT1G18270 AT1G24793 AT4G32820 At5g63440

EMBL accession:

BH850187

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.