NASC

NASC ID: N571348

Name: SALK_071348

ABRC stock number: SALK_071348

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At1g77480. T5M16.7 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_071348.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G77480.1 (at chr 1 pos 29114999 (C/29114999-29115074) on the TAIR10). The sequences' 1-76 bps mapped on the genome. Pools P 48, R 43, C 44 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393205.

SALKseq_071348.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G28110.2=P355 and AT1G28120.1=UTR3 (around chr 1 pos 9811940 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415878.

SALKseq_071348.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G50400.1=UTR3 and AT3G50410.1=P355 (at chr 3 pos 18708209 (W/18708134-18708209) on the TAIR10). The sequences' 1-76 bps mapped on the genome. Pools P 48, R 43, C 44 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399888.

SALKseq_071348.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G00891=P355 and AT4G00893.1=UTR3 (at chr 4 pos 378795 (W/378720-378795) on the TAIR10). The sequences' 1-76 bps mapped on the genome. Pools P 48, R 43, C 44 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403232.

SALKseq_071348.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G37460.1 (at chr 5 pos 14860437 (W/14860401-14860437) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 48, R 43, C 44 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406560.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_071348

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662907 (SALK_071348C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G28110 AT1G28120 AT3G50400 AT3G50410 AT4G00891 AT4G00893 AT5G37460 At1g77480

EMBL accession:

BH850489

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.