NASC Stock Detail Page
NASC ID: N571453
Name: SALK_071453
ABRC stock number: SALK_071453
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At3g26310. F20C19.3 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_071453.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G26310.1 and AT3G26320.1 (at chr 3 pos 9642910 (W/9642879-9642910) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 29-52 bps onto the T-DNA. Pools P 48, R 46, C 43 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343013. SALKseq_071453.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G55920.1=P355 and AT5G55930.1=P355 (at chr 5 pos 22651081 (W/22651004-22651081) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 48, R 46, C 43 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346355. SALKseq_071453.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G44990.1 and AT1G45000.1=P355 (at chr 1 pos 17008397 (W/17008353-17008397) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 42-73 bps onto the T-DNA. Pools P 48, R 46, C 43 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409902. SALKseq_071453.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G12200.1=UTR3 and AT1G12210.1 (at chr 1 pos 4140593 (W/4140539-4140593) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 46-72 bps onto the T-DNA. Pools P 48, R 46, C 43 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413263.
Donation Date: 2002-01-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_071453
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N656631 (SALK_071453C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.