NASC Stock Detail Page
NASC ID: N571588
Name: SALK_071588
ABRC stock number: SALK_071588
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.July 2002 TAIR gene annotation - At4g08980. T3H13.1 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 Associated Polymorphisms: SALKseq_071588.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G10690.1=P355 and AT1G10700.1=P355 (at chr 1 pos 3552931 (W/3552876-3552931) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 59-76 bps onto the T-DNA. Pools P 48, R 45, C 41 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO343021. SALKseq_071588.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G24040.1 (at chr 2 pos 10224622 (W/10224576-10224622) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 53-70 bps onto the T-DNA. Pools P 48, R 45, C 41 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO346363. SALKseq_071588.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G05440.1 and AT3G05450.1 (at chr 3 pos 1573671 (W/1573605-1573671) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 48, R 45, C 41 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO349710. SALKseq_071588.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G30240.1 (at chr 4 pos 14810307 (C/14810307-14810382) on the TAIR10). The sequences' 1-76 bps mapped on the genome. Pools P 48, R 45, C 41 N 41. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO353065.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_071588
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653285 (SALK_071588C).
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.