NASC

NASC ID: N573108

Name: SALK_073108

ABRC stock number: SALK_073108

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - F15A18.70 Intron. At5g27610. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_073108.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G27610.1 (at chr 5 pos 9765206 (C/9765206-9765260) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 51-76 bps onto the T-DNA. Pools P 49, R 45, C 50 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376535.
SALKseq_073108.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G27606.1 and AT5G27610.1=P355 (at chr 5 pos 9763491 (W/9763434-9763491) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 57-76 bps onto the T-DNA. Pools P 49, R 45, C 50 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373187.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_073108

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_073108C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT5G27606 At5g27610

EMBL accession:

BH851524

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.