NASC

NASC ID: N576533

Name: SALK_076533

ABRC stock number: SALK_076533

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - F14I3.11 Intron. At1g50280. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_076533.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G33780.1=P355 and AT1G33785 (at chr 1 pos 12248294 (W/12248217-12248294) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 62, R 63, C 64 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416690.

SALKseq_076533.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G05480.3=UTR3 and AT3G05490.1=P355 (at chr 3 pos 1590103 (W/1590053-1590103) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 62, R 63, C 64 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420008.

SALKseq_076533.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G09370.1 (at chr 5 pos 2910560 (W/2910531-2910560) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 34-65 bps onto the T-DNA. Pools P 62, R 63, C 64 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423324.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_076533

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666464 (SALK_076533C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G33780 AT1G33785 AT3G05480 AT3G05490 AT5G09370 At1g50280

EMBL accession:

BH857248

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.