NASC

NASC ID: N577422

Name: SALK_077422

ABRC stock number: SALK_077422

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - MMB12.17 Exon. At3g19680. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_077422.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G19680.1 (at chr 3 pos 6841773 (W/6841715-6841773) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 57, R 60, C 57 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396687.

SALKseq_077422.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G44705 and AT3G44710.1=UTR3 (at chr 3 pos 16251330 (W/16251269-16251330) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 53-77 bps onto the T-DNA. Pools P 57, R 60, C 57 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393351.

SALKseq_077422.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G11470.1 (at chr 4 pos 6970048 (W/6969995-6970048) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 57, R 60, C 57 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400036.

SALKseq_077422.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16990.5=UTR3 and AT4G17000.1=UTR3 (at chr 4 pos 9566015 (W/9565981-9566015) on the TAIR10). The sequences' 1-35 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 57, R 60, C 57 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403377.

SALKseq_077422.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G66680.1=P355 and AT5G66690.1=P355 (at chr 5 pos 26624435 (C/26624435-26624478) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 57, R 60, C 57 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406705.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_077422

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N657403 (SALK_077422C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G44705 AT3G44710 AT4G11470 AT4G16990 AT4G17000 AT5G66680 AT5G66690 At3g19680

EMBL accession:

BH856995

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.