NASC

NASC ID: N579246

Name: SALK_079246

ABRC stock number: SALK_079246

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At3g23540. MDB19.2 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_079246.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G23540.1 (at chr 3 pos 8446090 (W/8446046-8446090) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 48-70 bps onto the T-DNA. Pools P 66, R 66, C 67 N 70. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO436995.

SALKseq_079246.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G47030.1 (at chr 2 pos 19325649 (W/19325590-19325649) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 66, R 66, C 67 N 70. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO427070.

SALKseq_079246.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G19508.1 (at chr 3 pos 6762562 (C/6762562-6762646) on the TAIR10). The sequences' 1-85 bps mapped on the genome, 81-100 bps onto the T-DNA. Pools P 66, R 66, C 67 N 70. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO430386.

SALKseq_079246.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G20500.1 (at chr 3 pos 7159368 (C/7159368-7159467) on the TAIR10). The sequences' 1-100 bps mapped on the genome. Pools P 66, R 66, C 67 N 70. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO433691.

SALKseq_079246.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G27350.1 (at chr 5 pos 9651503 (W/9651446-9651503) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 66, R 66, C 67 N 70. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO417123.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_079246

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666499 (SALK_079246C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G47030 AT3G19508 AT3G20500 AT5G27350 At3g23540

EMBL accession:

BH856748

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.