NASC

NASC ID: N580708

Name: SALK_080708

ABRC stock number: SALK_080708

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.February 2003 TAIR gene annotation - At2g02390 T16F16. 18 L300-5' T16F16. 18 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_080708.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G02390.1 (at chr 2 pos 628833 (C/628833-628897) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 67, R 68, C 64 N 66. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO437202.

SALKseq_080708.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G78510.1 (at chr 1 pos 29536211 (W/29536134-29536211) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 67, R 68, C 64 N 66. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO433898.

SALKseq_080708.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G11610.1 (at chr 4 pos 7015623 (W/7015564-7015623) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 67, R 68, C 64 N 66. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO417329.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_080708

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653317 (SALK_080708C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G78510 AT4G11610 At2g02390

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.