NASC

NASC ID: N581116

Name: SALK_081116

ABRC stock number: SALK_081116

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At2g27740. F15K20.16 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_081116.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G27740.1 (at chr 2 pos 11823376 (W/11823322-11823376) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 50-75 bps onto the T-DNA. Pools P 67, R 67, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424043.

SALKseq_081116.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G62560.1=UTR3 and AT1G62570.1=P355 (at chr 1 pos 23167360 (W/23167300-23167360) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 67, R 67, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420727.

SALKseq_081116.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G46790.2=UTR3 and AT2G46800.2=P355 (at chr 2 pos 19235560 (W/19235494-19235560) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 67, R 67, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427359.

SALKseq_081116.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G15140.1 (at chr 4 pos 8640473 (W/8640399-8640473) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 67, R 67, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430675.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_081116

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663099 (SALK_081116C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G62560 AT1G62570 AT2G46790 AT2G46800 AT4G15140 At2g27740

EMBL accession:

BH856247

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.