NASC Stock Detail Page
NASC ID: N581127
Name: SALK_081127
ABRC stock number: SALK_081127
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_081127.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G44720.1 (at chr 5 pos 18043341 (W/18043280-18043341) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 67, R 64, C 61 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417413. SALKseq_081127.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G55520.1 (at chr 1 pos 20728102 (W/20728045-20728102) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 67, R 64, C 61 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430677. SALKseq_081127.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G04270.1 (at chr 2 pos 1479782 (C/1479782-1479843) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 67, R 64, C 61 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433982. SALKseq_081127.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G04404.1 and AT4G04405.1 (at chr 4 pos 2168930 (W/2168899-2168930) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 67, R 64, C 61 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO437286.
Donation Date: 2003-01-31
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_081127
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663102 (SALK_081127C).
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.