NASC

NASC ID: N582155

Name: SALK_082155

ABRC stock number: SALK_082155

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.February 2003 TAIR gene annotation - At1g55805 F20N2.2 3 Exon F20N2.2 3 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_082155.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G55805.1 (at chr 1 pos 20859125 (W/20859080-20859125) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 70, R 67, C 64 N 62. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO417598.

SALKseq_082155.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G63950.1 and AT1G63960.1 (at chr 1 pos 23736890 (W/23736842-23736890) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 70, R 67, C 64 N 62. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO420915.

SALKseq_082155.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G34590.1=UTR3 and AT4G34600.1=P355 (at chr 4 pos 16524167 (W/16524107-16524167) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 70, R 67, C 64 N 62. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO424231.

SALKseq_082155.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G02160.1 (at chr 5 pos 426288 (W/426256-426288) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 30-50 bps onto the T-DNA. Pools P 70, R 67, C 64 N 62. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO427546.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_082155

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653322 (SALK_082155C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G63950 AT1G63960 AT4G34590 AT4G34600 AT5G02160 At1g55805

EMBL accession:

BZ761826

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.