NASC

NASC ID: N585094

Name: SALK_085094

ABRC stock number: SALK_085094

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At5g16220 T21H19.140 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_085094.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G16220.1 (at chr 5 pos 5300590 (C/5300590-5300667) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 79, C 75 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421474.

SALKseq_085094.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G10260.3 (at chr 3 pos 3173286 (C/3173286-3173341) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 76, R 79, C 75 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431420.

SALKseq_085094.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G25210.1=UTR3 and AT4G25220.1=P355 (at chr 4 pos 12920159 (W/12920105-12920159) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 76, R 79, C 75 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434727.

SALKseq_085094.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G14770.1 and AT4G14780.1=P355 (at chr 4 pos 8489584 (C/8489584-8489661) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 79, C 75 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438031.

SALKseq_085094.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G16620.1 (at chr 4 pos 9359851 (W/9359788-9359851) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 76, R 79, C 75 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418158.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_085094

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663162 (SALK_085094C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G10260 AT4G14770 AT4G14780 AT4G16620 AT4G25210 AT4G25220 At5g16220

EMBL accession:

BZ594699

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.