NASC Stock Detail Page
NASC ID: N585807
Name: SALK_085807
ABRC stock number: SALK_085807
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At5g18200. MRG7.16 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_085807.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G18200.1 (at chr 5 pos 6015174 (W/6015097-6015174) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 70, R 62, C 63 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438161. SALKseq_085807.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G35320.1 (at chr 1 pos 12959255 (W/12959187-12959255) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 70, R 62, C 63 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428235. SALKseq_085807.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G50310.1 (at chr 1 pos 18636349 (W/18636288-18636349) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 70, R 62, C 63 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431550. SALKseq_085807.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G22780.1=P355 and AT2G22790.1 (at chr 2 pos 9694367 (W/9694333-9694367) on the TAIR10). The sequences' 1-35 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 70, R 62, C 63 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434857.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_085807
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_085807C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.