NASC Stock Detail Page
NASC ID: N587067
Name: SALK_087067
ABRC stock number: SALK_087067
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2003 TAIR gene annotation -At5g11330 F2I11.220 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_087067.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G11340.1 (at chr 5 pos 3618915 (C/3618915-3618992) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 75, R 77, C 79 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438398. SALKseq_087067.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G33612.1 (at chr 1 pos 12192622 (W/12192560-12192622) on the TAIR10). The sequences' 2-64 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools: P 75, R 77, C 79 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428471. SALKseq_087067.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G19110.1 (at chr 1 pos 6603319 (W/6603269-6603319) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 75, R 77, C 79 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431787. SALKseq_087067.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G60790.1=P355 and AT5G60800.2=UTR3 (at chr 5 pos 24460560 (C/24460560-24460637) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 75, R 77, C 79 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435094. SALKseq_087067.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G25920.1=P355 and AT5G25930.1=P355 (at chr 5 pos 9049793 (W/9049720-9049793) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 75, R 77, C 79 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418525.
Donation Date: 2003-04-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_087067
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N656721 (SALK_087067C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.