NASC Stock Detail Page
NASC ID: N587240
Name: SALK_087240
ABRC stock number: SALK_087240
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - F25A4.29 Intron, At1g74740. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_087240.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G74740.1 (at chr 1 pos 28081729 (W/28081698-28081729) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 75, R 73, C 78 N 74. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO431823. SALKseq_087240.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G64690.1 (at chr 1 pos 24038543 (W/24038481-24038543) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 75, R 73, C 78 N 74. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO425193. SALKseq_087240.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G64700.1 (at chr 1 pos 24040847 (C/24040847-24040911) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 75, R 73, C 78 N 74. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO428507.
Donation Date: 2002-01-09
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_087240
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663206 (SALK_087240C).
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.