NASC Stock Detail Page
NASC ID: N589114
Name: SALK_089114
ABRC stock number: SALK_089114
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_089114.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G13560.1 (at chr 3 pos 4428283 (W/4428229-4428283) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 49-73 bps onto the T-DNA. Pools P 74, R 79, C 74 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418909. SALKseq_089114.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G68710.1=P355 and AT1G68720.1=P355 (at chr 1 pos 25799741 (C/25799741-25799797) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 74, R 79, C 74 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432171. SALKseq_089114.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G33880.1=P355 and AT2G33980.1=UTR3 (at chr 2 pos 14345747 (W/14345670-14345747) on the TAIR10). The sequences' 1-78 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 74, R 79, C 74 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435478. SALKseq_089114.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G06180.1 (at chr 2 pos 2421552 (W/2421490-2421552) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 74, R 79, C 74 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438781. SALKseq_089114.6 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G47930.1 (at chr 5 pos 19406964 (C/19406964-19407014) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 64-90 bps onto the T-DNA. Pools P 74, R 79, C 74 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422225.
Donation Date: 2003-01-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_089114
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_089114C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.