NASC Stock Detail Page
NASC ID: N593800
Name: SALK_093800
ABRC stock number: SALK_093800
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - T6L1.12 Exon, At1g68940. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_093800.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G68940.2 (at chr 1 pos 25923197 (W/25923138-25923197) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 78, R 73, C 79 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419528. SALKseq_093800.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G46950.1 (at chr 2 pos 19291927 (W/19291850-19291927) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 78, R 73, C 79 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422846. SALKseq_093800.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G28660.1 (at chr 3 pos 10741163 (W/10741116-10741163) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 78, R 73, C 79 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426163. SALKseq_093800.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G28885.1 and AT5G28886.1 (at chr 5 pos 10899789 (W/10899724-10899789) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 78, R 73, C 79 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429477.
Donation Date: 2002-01-09
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_093800
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663365 (SALK_093800C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.