NASC

NASC ID: N611041

Name: SALK_111041

ABRC stock number: SALK_111041

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At3g61380 F2A19.4 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_111041.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G52600.1=UTR3 and AT1G52610.1 (at chr 1 pos 19594176 (C/19594176-19594235) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 20, R 11, C 15 N 18. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO363277.

SALKseq_111041.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G67455.1 (at chr 1 pos 25268513 (W/25268460-25268513) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 49-79 bps onto the T-DNA. Pools P 20, R 11, C 15 N 18. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366624.

SALKseq_111041.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G25610.1 and AT3G25620.1=UTR3 (at chr 3 pos 9313962 (W/9313902-9313962) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 20, R 11, C 15 N 18. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369969.

SALKseq_111041.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G07740.1 (at chr 5 pos 2459502 (W/2459441-2459502) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 20, R 11, C 15 N 18. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373327.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_111041

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_111041C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G52600 AT1G52610 AT1G67455 AT3G25610 AT3G25620 AT5G07740 At3g61380

EMBL accession:

BZ665337

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.