NASC Stock Detail Page
NASC ID: N623055
Name: SALK_123055
ABRC stock number: SALK_123055
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At5g28290 T8M17.60 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_123055.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G28290.1 (at chr 5 pos 10280632 (C/10280632-10280711) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 78-100 bps onto the T-DNA. Pools P 53, R 60, C 58 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413575. SALKseq_123055.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G16310.1 (at chr 4 pos 9221614 (C/9221614-9221665) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 49-74 bps onto the T-DNA. Pools P 53, R 60, C 58 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406880. SALKseq_123055.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G56310.1 (at chr 5 pos 22802325 (C/22802325-22802395) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 72-99 bps onto the T-DNA. Pools P 53, R 60, C 58 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343320. SALKseq_123055.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G17840.1 (at chr 4 pos 9918820 (C/9918820-9918876) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 55-100 bps onto the T-DNA. Pools P 53, R 60, C 58 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410218. SALKseq_123055.201 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G60570.1=UTR3 and AT5G60580.3=P355 (at chr 5 pos 24350233 (W/24350188-24350233) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 53, R 60, C 58 N 55. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346667.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_123055
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655906 (SALK_123055C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.