NASC

NASC ID: N625327

Name: SALK_125327

ABRC stock number: SALK_125327

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At5g66680 MSN2.7 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_125327.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G66680.1 (at chr 5 pos 26620514 (W/26620436-26620514) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 55, R 54, C 55 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386951.

SALKseq_125327.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G40275 (at chr 5 pos 16098662 (C/16098662-16098727) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 55, R 54, C 55 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383608.

SALKseq_125327.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G23170.1=P355 and AT5G23180.1 (at chr 5 pos 7801379 (C/7801379-7801452) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 55, R 54, C 55 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390297.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_125327

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_125327C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT5G23170 AT5G23180 AT5G40275 At5g66680

EMBL accession:

BZ383238

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.