NASC Stock Detail Page
NASC ID: N625436
Name: SALK_125436
ABRC stock number: SALK_125436
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At3g56670 T5P19.320 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_125436.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G56670.1 (at chr 3 pos 20989260 (C/20989260-20989325) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 55, R 56, C 57 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410348. SALKseq_125436.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G80410.1 (at chr 1 pos 30230746 (W/30230684-30230746) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 55, R 56, C 57 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407010. SALKseq_125436.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G26710.1=UTR3 and AT3G26720.1=P355 (at chr 3 pos 9815110 (W/9815050-9815110) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 55, R 56, C 57 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413704. SALKseq_125436.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT4G35720.1 (at chr 4 pos 16929136 (W/16929103-16929136) on the TAIR10). The sequences' 1-34 bps mapped on the genome, 31-79 bps onto the T-DNA. Pools P 55, R 56, C 57 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343451. SALKseq_125436.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G13520.1 (at chr 5 pos 4343073 (W/4343003-4343073) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 55, R 56, C 57 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346798.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_125436
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_125436C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.