NASC

NASC ID: N626090

Name: SALK_126090

ABRC stock number: SALK_126090

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At1g19270 T29M8.14 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_126090.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G80570.3=UTR3 and AT1G80580.1 (at chr 1 pos 30292450 (W/30292351-30292450) on the TAIR10). The sequences' 1-100 bps mapped on the genome. Pools P 55, R 60, C 58 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO370247.

SALKseq_126090.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G46340.1=P355 and AT2G46360.1=P355 (at chr 2 pos 19027888 (W/19027834-19027888) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 55, R 60, C 58 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373605.

SALKseq_126090.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G58700.1=UTR3 and AT3G58710.1=P355 (at chr 3 pos 21713098 (C/21713098-21713151) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 55, R 60, C 58 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376952.

SALKseq_126090.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G02950.1 (at chr 5 pos 692659 (W/692581-692659) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 55, R 60, C 58 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380300.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_126090

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N667256 (SALK_126090C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G80570 AT1G80580 AT2G46340 AT2G46360 AT3G58700 AT3G58710 AT5G02950 At1g19270

EMBL accession:

BZ764670

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.