NASC Stock Detail Page
NASC ID: N626568
Name: SALK_126568
ABRC stock number: SALK_126568
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At5g07170 T28J14.110 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_126568.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G28465.1 (at chr 1 pos 10007437 (W/10007408-10007437) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 32-76 bps onto the T-DNA. Pools P 58, R 56, C 53 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO363580. SALKseq_126568.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G04020.1 and AT5G04030.1 (at chr 5 pos 1087491 (C/1087491-1087542) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 58, R 56, C 53 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366930. SALKseq_126568.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G38710.1=UTR3 and AT5G38720.1=UTR3 (at chr 5 pos 15505788 (C/15505788-15505840) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 58, R 56, C 53 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO370274. SALKseq_126568.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G40260.1 (at chr 5 pos 16090784 (C/16090784-16090862) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 58, R 56, C 53 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373630.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_126568
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_126568C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.