NASC Stock Detail Page
NASC ID: N627518
Name: SALK_127518
ABRC stock number: SALK_127518
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At2g05520 T20G20.13 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_127518.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G05520.1 (at chr 2 pos 2025948 (W/2025876-2025948) on the TAIR10). The sequences' 5-77 bps mapped on the genome, 73-95 bps onto the T-DNA. Pools P 57, R 53, C 57 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356927. SALKseq_127518.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G05635.1 and AT2G05640.1 (at chr 2 pos 2090994 (C/2090994-2091043) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 57, R 53, C 57 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360278. SALKseq_127518.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G45730.1 (at chr 5 pos 18552754 (W/18552688-18552754) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 56-86 bps onto the T-DNA. Pools P 57, R 53, C 57 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO363627. SALKseq_127518.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G67160.1 (at chr 5 pos 26798073 (C/26798073-26798151) on the TAIR10). The sequences' 1-79 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 57, R 53, C 57 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366978.
Donation Date: 2003-01-31
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_127518
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_127518C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.