NASC Stock Detail Page
NASC ID: N630095
Name: SALK_130095
ABRC stock number: SALK_130095
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_130095.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G06050.1 (at chr 2 pos 2362506 (C/2362506-2362556) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 51, R 53, C 58 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400565. SALKseq_130095.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G45000.2 and AT3G45010.1=P355 (at chr 3 pos 16462757 (C/16462757-16462818) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 51, R 53, C 58 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403907. SALKseq_130095.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G51680.1 and AT3G51690.1=UTR3 (at chr 3 pos 19176175 (C/19176175-19176253) on the TAIR10). The sequences' 1-78 bps mapped on the genome, 71-95 bps onto the T-DNA. Pools P 51, R 53, C 58 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407238. SALKseq_130095.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G35725 and AT5G35730.1=P355 (at chr 5 pos 13892001 (C/13892001-13892071) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 51, R 53, C 58 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410582.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_130095
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663988 (SALK_130095C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.