NASC

NASC ID: N630487

Name: SALK_130487

ABRC stock number: SALK_130487

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g50810 F18B3.9 0 Intron F18B3.9 0 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_130487.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G50808.1 (at chr 3 pos 18887989 (W/18887938-18887989) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 25, R 21, C 25 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO370462.

SALKseq_130487.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G60400.1 and AT1G60410.1=P355 (at chr 1 pos 22258002 (W/22257953-22258002) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 48-71 bps onto the T-DNA. Pools P 25, R 21, C 25 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO367113.

SALKseq_130487.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G57410.1 (at chr 3 pos 21248705 (C/21248705-21248737) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 30-50 bps onto the T-DNA. Pools P 25, R 21, C 25 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373814.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_130487

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663993 (SALK_130487C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G60400 AT1G60410 AT3G50808 AT3G57410 At3g50810

EMBL accession:

BZ357268

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.