NASC Stock Detail Page
NASC ID: N631274
Name: SALK_131274
ABRC stock number: SALK_131274
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At3g05240 T12H1.2 1 Exon T12H1.2 1 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_131274.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G05240.1 (at chr 3 pos 1493791 (W/1493721-1493791) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 65-83 bps onto the T-DNA. Pools P 25, R 20, C 24 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340380. SALKseq_131274.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G05240.1 (at chr 3 pos 1493833 (C/1493833-1493900) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 66-83 bps onto the T-DNA. Pools P 25, R 20, C 24 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340763. SALKseq_131274.1 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G19580.1 (at chr 5 pos 6608989 (C/6608989-6609037) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 53-76 bps onto the T-DNA. Pools P 25, R 28, C 26 N 25. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429879.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_131274
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664013 (SALK_131274C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.