NASC

NASC ID: N637516

Name: SALK_137516

ABRC stock number: SALK_137516

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.February 2003 TAIR gene annotation - At1g10810 T16B5.5 I ntron T16B5.5 I ntron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_137516.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G10810.1 (at chr 1 pos 3600118 (W/3600077-3600118) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 50-76 bps onto the T-DNA. Pools P 41, R 49, C 47 N 49. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO394220.

SALKseq_137516.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G60670.1 (at chr 1 pos 22347430 (C/22347430-22347490) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 41, R 49, C 47 N 49. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO390866.

SALKseq_137516.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G61960.2=P355 and AT3G61962.1 (at chr 3 pos 22945947 (W/22945885-22945947) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 41, R 49, C 47 N 49. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO397560.

SALKseq_137516.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G63570.1=UTR3 and AT5G63580.1 (at chr 5 pos 25454111 (C/25454111-25454169) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 41, R 49, C 47 N 49. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO400905.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_137516

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653512 (SALK_137516C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G60670 AT3G61960 AT3G61962 AT5G63570 AT5G63580 At1g10810

EMBL accession:

BZ766519

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.