NASC

NASC ID: N638399

Name: SALK_138399

ABRC stock number: SALK_138399

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.February 2003 TAIR gene annotation - At3g46450 F18L15. 17 0 Exon F18L15. 17 0 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_138399.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G01950.1 (at chr 2 pos 442365 (C/442365-442411) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 77, R 71, C 73 N 72. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO347413.

SALKseq_138399.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G25220.1 (at chr 3 pos 9182656 (W/9182606-9182656) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 53-77 bps onto the T-DNA. Pools P 77, R 71, C 73 N 72. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO350758.

SALKseq_138399.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G20780.1 (at chr 4 pos 11134127 (C/11134127-11134204) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 77, R 71, C 73 N 72. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354100.

SALKseq_138399.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G20790.1 and AT4G20800.1=P355 (at chr 4 pos 11137749 (W/11137672-11137749) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 77, R 71, C 73 N 72. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO357429.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_138399

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653515 (SALK_138399C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G01950 AT3G25220 AT4G20780 AT4G20790 AT4G20800 At3g46450

EMBL accession:

BZ767128

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.