NASC Stock Detail Page
NASC ID: N638477
Name: SALK_138477
ABRC stock number: SALK_138477
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. A HOMOZYGOUS LINE EXISTS FOR THIS STOCK. TO VIEW IT AND ORDER CLICK ON THE VIEW SUB LINES LINK BELOW. February 2003 TAIR gene annotation - At2g18590 F24H14. 6 Exon F24H14. 6 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_138477.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G18590.1 (at chr 2 pos 8072283 (W/8072256-8072283) on the TAIR10). The sequences' 1-28 bps mapped on the genome, 40-70 bps onto the T-DNA. Pools P 41, R 41, C 46 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO357433. SALKseq_138477.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G27620.1 (at chr 3 pos 10230882 (W/10230805-10230882) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 41, R 41, C 46 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360783. SALKseq_138477.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G25585.4=UTR3 and AT3G25590.1=P355 (at chr 3 pos 9299419 (C/9299419-9299451) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 55-72 bps onto the T-DNA. Pools P 41, R 41, C 46 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO364136. SALKseq_138477.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G22791.2=P355 and AT5G22792 (at chr 5 pos 7605434 (C/7605434-7605505) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 41, R 41, C 46 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO367483.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_138477
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N659247 (SALK_138477C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.