NASC Stock Detail Page
NASC ID: N638702
Name: SALK_138702
ABRC stock number: SALK_138702
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At2g15870 F19G14. 13 R300-5' F19G14. 13 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_138702.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G32280.1 and AT1G32290.1 (at chr 1 pos 11648151 (C/11648151-11648219) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 23, R 29, C 21 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO384237. SALKseq_138702.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G41350.1 (at chr 2 pos 17233139 (C/17233139-17233197) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 23, R 29, C 21 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387587. SALKseq_138702.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G04870.1 (at chr 3 pos 1343139 (W/1343099-1343139) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 39-72 bps onto the T-DNA. Pools P 23, R 29, C 21 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390928. SALKseq_138702.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G05020.1 (at chr 4 pos 2576201 (W/2576148-2576201) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 51-79 bps onto the T-DNA. Pools P 23, R 29, C 21 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO394281.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_138702
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_138702C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.