NASC

NASC ID: N639265

Name: SALK_139265

ABRC stock number: SALK_139265

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At5g59880 MMN10.4 I ntron MMN10.4 I ntron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_139265.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G59880.1 (at chr 5 pos 24120403 (W/24120365-24120403) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 43-78 bps onto the T-DNA. Pools P 39, R 33, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407669.

SALKseq_139265.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G01580.1=UTR3 and AT1G01590.1 (at chr 1 pos 213645 (W/213589-213645) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 39, R 33, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397652.

SALKseq_139265.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G13181.1 and AT5G13190.1=UTR3 (at chr 5 pos 4199376 (W/4199318-4199376) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 39, R 33, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO411014.

SALKseq_139265.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G37210.1=P355 and AT2G37220.1=UTR3 (at chr 2 pos 15633208 (W/15633147-15633208) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 39, R 33, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400994.

SALKseq_139265.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G22470.1=P355 and AT2G22475.1=P355 (at chr 2 pos 9540150 (C/9540150-9540195) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 39, R 33, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO404330.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_139265

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664149 (SALK_139265C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G01580 AT1G01590 AT2G22470 AT2G22475 AT2G37210 AT2G37220 AT5G13181 AT5G13190 At5g59880

EMBL accession:

BZ767722

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.