NASC Stock Detail Page
NASC ID: N639266
Name: SALK_139266
ABRC stock number: SALK_139266
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_139266.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G59640.1 (at chr 1 pos 21911567 (W/21911505-21911567) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 38, R 39, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO414363. SALKseq_139266.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G71360.1 (at chr 1 pos 26894803 (W/26894741-26894803) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 38, R 39, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO344112. SALKseq_139266.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G10300.1 and AT1G10310.1=UTR3 (at chr 1 pos 3381551 (W/3381498-3381551) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 38, R 39, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO347459. SALKseq_139266.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G25760.1 (at chr 2 pos 10988901 (W/10988847-10988901) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 38, R 39, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350804. SALKseq_139266.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G26890.1 (at chr 2 pos 11463152 (W/11463116-11463152) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 47-78 bps onto the T-DNA. Pools P 38, R 39, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO354145.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_139266
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654429 (SALK_139266C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.