NASC Stock Detail Page
NASC ID: N70471
Name: JYB1221.3
ABRC stock number: CS70471
Description: Transgenic GFP-fusion expression line harboring the pE22::cE22:GFP(-G):NOS construct. The GFP reporter fusion construct was generated by using the MultiSite Gateway Three-Fragment Vector Construction system (Invitrogen); final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants (T1) were selected with Basta on soil and 5 to 7-day-old T2 seedlings were grown and imaged by using confocal laser scanning microscopy; distributed seeds are T3.
Donation Date: 2008-08-22
| Donated by: | Duke University Philip Benfey Cornell University Ji-Young Lee |
Donor Number: JYB1221.3
Part of : Benfey GFP-fusion expression lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: Col(Columbia)
Phenotype
Transcription factor (TF) expressed in a tissue-enriched manner in roots: spotty GFP in stele from maturation zone, more concentrated around pericycle (5/6; 4DAG).
References
Lee, J.Y. et al. 2006. Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots. Proceedings of the National Academy of Sciences of the United States of America 103(15):6055-60.PubMed ID: 16581911.