Home | About NASC | Address/Staff
Ask a question | Help

NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N802464

Name: SAIL_52_E04

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:
SAIL_52_E04 : Genbank Annotation: 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G19410.1 and AT2G19420.1 (at chr 2 pos 8410446 (W/8410380-8410446) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 61-95 bps onto the T-DNA. Pools P 14, R 12, C 12 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO330775.

Donation Date: 2004-06-01

Donated by:  Syngenta AG Alberto Martinez

Donor Number: SAIL_52_E04

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SAIL_52_E04.

Status Price (£)
Available to order at NASC £7.50

Germplasm Info

Background: Col-3( N8846)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

McElver, J. et al. 2001. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159(4):1751-63.PubMed ID: 11779812.