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NASC Stock Detail Page

NASC ID: N828822

Name: SAIL_662_D10

ABRC stock number: CS828822

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:
SAIL_662_D10 : Genbank Annotation: 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G65470.1 (at chr 1 pos 24321762 (W/24321684-24321762) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 30, R 28, C 23 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO333242.

Donation Date: 2004-06-01

Donated by:  Syngenta AG Alberto Martinez

Donor Number: SAIL_662_D10

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Status Price (£)
Available £10.00

Germplasm Info

Background: Col-0( N60000)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • FAS1
  • NFB2
EMBL accession:

References

McElver, J. et al. 2001. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159(4):1751-63.PubMed ID: 11779812.