NASC

NASC ID: N849007

Name: WiscDsLox1E7

ABRC stock number: CS849007

Description: Sequence-indexed T-DNA insertion line; the T-DNA includes a Ds transposon and a LoxP recombination site. A Basta resistance marker is used to select for the T-DNA and for the Ds transposon following any transposition. However, be aware that lines from this set might not be able to launch their Ds element. Be sure to test any line for the ability to transpose before using it in experiments. Hygromycin resistance can be used for selection of lines where the Ds element has been excised by transposition. WiscDsLox lines do not contain Ac transposase, and the lines should be crossed to a line expressing Ac transposase in order to mobilize the Ds transposon. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Associated Polymorphisms:

Wiscseq_DsLox1E7.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G02620.1-UTR3 and AT1G02630.1-UTR3 (at chr 1 pos 560284 (C/560284-560366) on the TAIR10). The sequences' 1-83 bps mapped on the genome, 80-100 bps onto the T-DNA. Pools P 0, R 0, C 3 N 0. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452072.

Wiscseq_DsLox1E7.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G34500.1 (chr 2 pos 14539505 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG795866.

Wiscseq_DsLox1E7.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G09520.1 (chr 3 pos 2924587 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795867.

Wiscseq_DsLox1E7.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G13500.1 (chr 5 pos 4337968 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795868.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox1E7

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G02620 AT1G02630 AT2G34500 AT3G09520 AT5G13500