NASC

NASC ID: N849014

Name: WiscDsLox1F2

ABRC stock number: CS849014

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox1F2.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G20810.1 (chr 2 pos 8957636 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795873.

Wiscseq_DsLox1F2.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G10370.1-P355 and AT1G10380.1-P355 (at chr 1 pos 3398950 (C/3398950-3399013) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 84-100 bps onto the T-DNA. Pools P 0, R 0, C 3 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452673.

Wiscseq_DsLox1F2.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G23080.2 (chr 3 pos 8209325 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795874.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox1F2

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox1F2	WiscDsLox1F2	AT1G10370 AT1G10380 AT3G23080 At2g20810