NASC

NASC ID: N855625

Name: WiscDsLox434B7

ABRC stock number: CS855625

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox434B7 .

Wiscseq_DsLox434B7.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G80640.1 (chr 1 pos 30314162 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG804741.

Wiscseq_DsLox434B7.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G22770.1 (chr 4 pos 11964800 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG804742.

Wiscseq_DsLox434B7.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G48340.1 (chr 5 pos 19592804 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG804743.

Wiscseq_DsLox434B7.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G64401.1 (chr 5 pos 25750535 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG804744.

Donation Date: 2006-09-07

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox434B7

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox434B7	WiscDsLox434B7	AT1G80640 AT4G22770 AT5G48340 AT5G64401 At1g80650

EMBL accession:

CW796313