NASC

NASC ID: N924305

Name: Wisc_DsLox240F05.2

ABRC stock number: CS924305

Description: confirmed line isolated from original WISC line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.

Donation Date: 2015-06-19

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: Wiscseq_DsLox240F05.2

Stock type: individual line

Material type: seed

Comment: Donors: Joseph R. Ecker. Clone Name: pDs-Lox. 2015-02-18 GenBank annotation - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G40390.1 (at chr 5 pos 16161525 (C/16161525-16161624) on the TAIR10). The sequences' 1-100 bps mapped on the genome, 14-28 bps onto the T-DNA. Pools: P 0, R 4, C 4 N 3. The insertion site is at the 3' end of the sequence mapping. GenBank Acc: KO453288

Status	Price (£)
Available	£11.00

Germplasm Info

Background: Col(Columbia), WiscDsLox240F05 ( N849460)

Growth requirement: none

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT5G40390