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NASC ID: N9561

Name: PtGFP_kn

ABRC stock number: CS9561

Description: Pt-GFP is a pH-reporter protein when used ratiometrically in the double excitation mode. It has a broad pH-responsiveness and a good acid stability.Arabidopsis lines PtGFP_Kn and PtGFP6-3 can be used to monitor cytoplasmic pH in a wide range of applications. PtGFP_kn and PtGFP6-3 represent two expression lines from two different floral dip experiments. Hence the T-DNA from the Agrobacs carrying the PtGFP gene has been inserted in different (as yet not identified) locations of the Arabidopsis genome, leading to different expression levels (kn appears a bit brighter than 6-3).

Donation Date: 2006-03-31

Donated by:  Christian-Albrechts-Universit√§t zu Kiel Christoph Plieth

Donor Number: PtGFP_kn

Stock type: individual line

Material type: seed

Comment: related stock PtGFP6-3 . Further information is found at: http://www.nanolight.com/nanofluor.htm

Status Price (£)
Available £10.00

Germplasm Info

Seed type: Single marker

Background: Col-0 ( N1092 )

Marker used: green fluorescent protein from Ptilosarcus gurneyi (seapen)

Generation: F2

Segregation: yes

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

EMBL accession:

Phenotype

These lines express green fluorescent protein from the seapen Ptilosarcus gurneyi (Pt-GFP) constitutively in the cytoplasm under control of a single 35S-CaMV-promoter.Cells fluoresce green when exited with blue light.There are no other differences in phenotype neither in appearance and growth nor in development and reproduction when compared to Col-0 wild type.

References

Schulte, A., et al. 2006. A novel pH indicator for expression in plants. Plant methods 2:7.PubMed ID: 16600023.