NASC Stock Detail Page
NASC ID: N9984
Name: Noccaea caerulescens Saint Laurent Le Minier A7M5
ABRC stock number: CS9984
Description: 5500 M0 seeds were irradiated with fast neutrons for 137 min, at a dose rate of 60 Gy (Biological Irradiation Facility, Budapest Research Reactor (BRR), Budapest Neutron Centre, Budapest, Hungary). The mean water kerma dose rate was monitored in real time by U-235 and Th-232 fission chambers and a Geiger–Mu¨ller counter and was 438 mGy min-1 (± 3.0%). The BRR tank-type reactor, with a nominal thermal power of 10 MW, was moderated and cooled by light water. Filters of different densities were arranged in order to decrease gamma and neutron intensity and to modify the neutron spectrum and the neutron : gamma ratio. The filters were set at the following distances from the core towards the irradiation cavity: 143.6 mm Al + 18 mm Pb + 15 mm Al. At the beam stop behind the sample, shielding comprised 30 mm Fe + 45 mm Pb + 8 mm Al + 20 mm B4C. Seeds were irradiated inside a rotating Cd capsule (16 rpm) of 2 mm wall thickness at a temperature < 30_C and humidity < 70%, at normal air pressure. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost. After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. Following vernalization of young M1 plants, 79% of plants survived to maturity. In all, 80 000 M2 lines were screened for flowering in the absence of vernalization. Floral initials were observed in 35 lines, with A7 flowering in < 12 wk and selfed to the M5 without vernalization.
Donation Date: 2011-01-11
| Donated by: | University of Nottingham Martin Broadley University of Nottingham Seosamh O'Lochlainn University of Nottingham Neil Graham |
Donor Number: Noccaea caerulescens Saint Laurent Le Minier A7M5
Part of : Noccaea caerulescens mutants/ecotypes
Stock type: individual line
Material type: seed
Comment: formerly known as Noccaea caerulescens
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Genus: Thlaspi Species: caerulescens
Seed type: Mutant
Mutagen: fast neutrons
Background: Thlaspi caerulescens
Segregation status: homozygous
Growth requirement: Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively, in 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Lev
Phenotype
Early Flowering (within 2 months) and early fruiting (between 3-4 months) nonvernal obligate mutant lines, selfed to M5
Ecotype data
Location: Saint-Laurent-Le-Minier, Ganges
Habitat: Heavy-metal-enriched, Zn-Pb-Cd, lead/zinc mine spoil tailings
Country: FRANCE
Altitude Min: 100
Altitude Max: 500
Latitude Min: 43.93
Latitude Max: 43.93
Longitude Min: 3.67
Longitude Max: 3.67
References
Lochlainn, S. et al. 2011. Generation of non vernal-obligate, faster-cycling Noccaea caerulescens lines through fast neutron mutagenesis. New Phytologist 189(2):409-14.PubMed ID: 21058953.