NASC Stock Detail Page
NASC ID: N16352
Name: rga-28
ABRC stock number: CS16352
Description: Line isolated by PCR screening the multidimensional DNA pools of T-DNA insertion mutant population generated in the Col-0 background at the Salk Institute. The T-DNA left border primer and degenerate primer 244 was used for PCR. Mutant line was identified by analyzing PCR products by DNA-blot analyses using gene-specific cDNA probe for the gene of interest, and the location of the T-DNA insertion was determined by DNA sequence analysis of the PCR products. Homozygous mutant plants were identified by PCR using allele-specific primers. To detect any remaining transcript produced, RT-PCR was performed using primers that span the T-DNA insertion and are specific for sequences upstream of the insertion.
Donation Date: 2008-08-21
Donated by: | Duke University Tai-ping Sun |
Donor Number: rga-28
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Columbia (N60000)
Segregation status: homozygous
Pedigree: Backcrossed once to wild type.
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
No visible phenotype.
References
Tyler, L. et al. 2004. Della proteins and gibberellin-regulated seed germination and floral development in Arabidopsis. Plant physiology 135(2):1008-19.PubMed ID: 15173565.