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The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N1695

Name: CHS silencing line C

ABRC stock number: CS1695

Description: Reduced anthocyanin production under inducing conditions such as high light intensity or growth in 2% sucrose. Bleaching of inflorescence under high light (sodium lamps or sunlight).

Donation Date: 1997-09-21

Donated by:  University of Cambridge Ian Furner

Donor Number: Line C

Part of : Homozygous lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Ler

Segregation status: homozygous

Pedigree: Transformed Ler backcrossed to Ler and Colombia (latter containing the clv1-2 mutation).

Transformation method: Agrobacterium mediated transformation following the method of Valvekans et al (1988) Proc. Natl. Acad.Sci. USA, 85, 5536-5540.

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

Phenotype

Transgenic showing CHS silencing - Line C. Homozygous. Little anthocyanin. Yellow seed. Little Kanamycin or Hygromycin resistance. Also homozygous for clv1-2.

References

Davies, G.J. et al. 1997. Genetics of homology-dependent gene silencing in Arabidopsis; a role for methylation. The Plant Journal 12(4):791-804.PubMed ID: 9375393.