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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N3875

Name: resistant to NPA

ABRC stock number: CS3875

Description: Biochemical, Form

Donation Date: 2001-08-16

Donated by:  Brown University Alison DeLong

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Ws (Wassilewskija)

Marker used: Kanamycin segregation Resistant:Senstive(Yes)

Segregation: No

Transformation method: insertional mutagenesis, Agrobacterium transformation

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • rcn1-1
  • RCN1

Phenotype

decreased root and hypocotyl elongation in dark grown seedlings; apical hypocotyl hook defect (a partial hook forms); irregular waving and reversed slant in root waving assay; increased sensitivity to phosphatase inhibitors cantharidin and okadaic acid; encodes an A regulatory subunit of protein phosphatase 2A; homozygous for kanamycin resistance; backcrossed 3 times to Ws. Homozygous

References

Deruere, J. 1999.The RCN1-encoded a subunit of protein phosphatase 2A increasesphosphatase activity in vivo. The Plant Journal 20(4): 389-399.PubMed ID: 10607292.