NASC Stock Detail Page
NASC ID: N3876
Name: sucrose transporter
ABRC stock number: CS3876
Description: Biochemical
Donation Date: 2001-08-16
| Donated by: | University of Wisconsin, Madison Jennifer Gottwald University of Wisconsin, Madison Michael Sussman |
Donor Number: suc2-1 12-7
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Ws (Wassilewskija)
Segregation: Yes
Transformation method: insertional mutagenesis, Agrobacterium transformation
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
mutant seedlings germinated on media without sucrose are smaller than wild type, have yellowing and translucent cotyledons, very short primary roots and no rosette leaves; do not develop beyond the cotyledon stage without sucrose, but can be partially rescued by the addition of sucrose. When germinated on media with supplemental sucrose, homozygotes do not appear different from wild-type or heterozygous seedlings; after transfer to soil with no exogenous sucrose available, homozygous mutant plants are dwarfed with delayed development; fewer rosette leaves which are darker green, very small, and shaped more like juvenile than adult leaves; plants may occasionally flower but never produce viable seed; encodes a phloem-specific sucrose transporter; maintained as heterozygote; backcrossed once to wild type.
References
Gottwald, J. et al. 2000. Genetic evidence for the in planta role of phloem specific plasmamenbrane sucrose transporters. Proceedings of the National Academy of Sciences of the United States of America 97(25):13979-84.PubMed ID: 11087840.